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Zidovudine

Catalog No. T1416 CAS 30516-87-1

Synonyms: AZT, Azidothymidine, ZDV, NSC 602670

Zidovudine is a synthetic dideoxynucleoside. After intracellular phosphorylation to its active metabolite, zidovudine inhibits DNA polymerase, resulting in the inhibition of DNA replication and cell death. This agent also decreases levels of available pyrimidines.

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Zidovudine, CAS 30516-87-1

Pack Size	Availability	Price/USD
50 mg	In stock	$ 47.00
100 mg	In stock	$ 58.00
200 mg	In stock	$ 76.00
500 mg	In stock	$ 130.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.91%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Zidovudine is a synthetic dideoxynucleoside. After intracellular phosphorylation to its active metabolite, zidovudine inhibits DNA polymerase, resulting in the inhibition of DNA replication and cell death. This agent also decreases levels of available pyrimidines.
In vivo	Intravitrous injection of the NRTIs Lamivudine (3TC), Zidovudine (AZT), or Abacavir (ABC) suppresses the laser-induced choroidal neovascularization (CNV) in wild-type mice compared to PBS vehicle. The mean level of VEGF-A in the RPE/choroid, which peaks on day 3 after laser injury, is significantly reduced in 3TC-, AZT- and ABC-treated eyes compared with control eyes in wild-type mice, but not inP2rx7-/- mice[6].
Cell Research	Zidovudine is prepared in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Assays are performed in all cell types in the presence of titrating concentrations of ARV. 5,000 SVG, 2,500 PFA, 200,000 PBMC, or 50,000 MDM cells/well are seeded into triplicate wells of 96-well plates. Twenty-four hours later, the culture medium is removed and replaced with medium containing the ARV or DMSO (0.5% vol/vol), and equivalent TCID50 infectious units of luciferase reporter virus are added to the cells. After a 16 h incubation at 37°C, the initial viral inoculum is removed and replaced with culture medium containing the same antiretroviral drug (ARV) or DMSO (0.5% vol/vol) concentrations. At 72 h post infection, the medium is aspirated, the cells are lysed and HIV-1 infection measured using the Luciferase Assay System. Luminescence is measured using a FLUOStar Optima microplate reader. Inhibition curves and the 50% (EC50) and 90% (EC90) effective concentrations are determined by nonlinear regression analysis, using GraphPad Prism software[1].

Synonyms	AZT, Azidothymidine, ZDV, NSC 602670
Molecular Weight	267.24
Formula	C10H13N5O4
CAS No.	30516-87-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 2 years

Solubility Information

H2O: 13.4 mg/mL (50 mM)

DMSO: 26.7 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Chiang G, et al. Clin Ther,1996, 18(6), 1080-1092. 2. Panther LA, et al. J Med Virol,1999, 58(2), 165-173. 3. Wang H, et al. Biochim Biophys Acta,1996, 1316(1), 51-59. 4. Chitnis S, et al. Life Sci,2002, 71(8), 967-978. 5. Gogu SR, et al. Biochem Pharmacol,1995, 50(3), 413-419. 6. Hou P, et al. Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection. Sci Rep. 2015 Oct 20;5:15577. 7. Mizutani T, et al. Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice. Invest Ophthalmol Vis Sci. 2015 Nov;56(12):7122-9. 8. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes[J]. Molecules. 2021, 26(8): 2123. 9. Tan S, Li W, Li Z, et al. A Novel CXCR4 Targeting Protein SDF-1/54 as an HIV-1 Entry Inhibitor. Viruses. 2019, 11(9): 874. 10. Tan S, Li J Q, Cheng H, et al. The anti-parasitic drug suramin potently inhibits formation of seminal amyloid fibrils and their interaction with HIV-1[J]. Journal of Biological Chemistry. 2019: jbc. RA118. 006797.

Citations

1. Tan S, Li J Q, Cheng H, et al. The anti-parasitic drug suramin potently inhibits formation of seminal amyloid fibrils and their interaction with HIV-1. Journal of Biological Chemistry. 2019: jbc. RA118. 006797 2. Tan S, Li W, Li Z, et al. A Novel CXCR4 Targeting Protein SDF-1/54 as an HIV-1 Entry Inhibitor. Viruses. 2019, 11(9): 874 3. Wu Y, Chu L, Yang H, et al. Simultaneous Determination of 6 Antiretroviral Drugs in Human Hair Using an LC-ESI+-MS/MS Method: Application to Adherence Assessment. Therapeutic Drug Monitoring. 2021, 43(6): 756-765. 4. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes. Molecules. 2021, 26(8): 2123. 5. Xu H, You H, Gong J, et al.Discovery of Zidovudine as a cardiomyocyte protectant for doxorubicin-induced toxicity through high-throughput phenotypic drug screening.Fundamental Research.2023

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Pediatric Drug Library Drug-Fragment Library Drug Repurposing Compound Library Anti-COVID-19 Compound Library Anti-Viral Compound Library FDA-Approved Drug Library Drug-induced Liver Injury (DILI) Compound Library Nucleotide Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Zidovudine 30516-87-1 DNA Damage/DNA Repair Microbiology/Virology Proteases/Proteasome CRISPR/Cas9 Telomerase Reverse Transcriptase HIV Protease AZT HIV Azidothymidine inhibit ZDV Human immunodeficiency virus Inhibitor NSC 602670 inhibitor

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