store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vinorelbine (KW-2307 base) is a semisynthetic vinca alkaloid. Vinorelbine binds to tubulin and prevents the formation of the mitotic spindle, resulting in the arrest of tumor cell growth in metaphase.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 mg | In stock | $ 90.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 70.00 |
Description | Vinorelbine (KW-2307 base) is a semisynthetic vinca alkaloid. Vinorelbine binds to tubulin and prevents the formation of the mitotic spindle, resulting in the arrest of tumor cell growth in metaphase. |
Targets&IC50 | proliferation(Hela cells):1.25 nM |
In vitro | Vinorelbine (0.5-5 nM) inhibits cell proliferation by 50% (IC50) at concentrations of 1.25 nM. At concentration of 8 nM vinorelbine, no cells are in anaphase[1]. Vinorelbine time-dependently induces the p53 and p21WAFI/CIP1 expression in androgen-dependent (AD) and- independent (AI) prostate cancer cell lines. Vinorelbine stimulates reporter genes in a concentration-dependent manner[2]. |
In vivo | After vinorelbine treatment, the first neutropenicepisode occurred after the first (4 dogs), second (1), or sixth(1) vinorelbine treatment in the dogs[3]. Vinorelbine is tolerated at a weekly interval in tumor-bearing cats, with an MTD of 11.5 mg/m2[4]. |
Kinase Assay | Reverse transcriptase assays: Reverse transcriptase assays are carried out in a reaction mixture (50 μL) containing 50 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM potassium chloride, 0.01% Triton X-100 or NP40, 10 μg/ml (dT)15.(A)n as template primer and [3H] deoxythymidine triphospahate. The reaction mixture is incubated for 1 hr at 37 ℃ and stopped by the addition of 50 μg of yeast tRNA and 2 mL of 10% solution of trichloroacetic acid containing 1 mM sodium pyrophosphate. The samples are filtered on filters (0.45 μm), washed first with 5% TCA solution for 5 times and then with 2 ml of 70% ethanol. The filters are dried, scintillation fluid is added and the radioactivity counted in a counter. |
Source |
Synonyms | KW-2307 base |
Molecular Weight | 778.93 |
Formula | C45H54N4O8 |
CAS No. | 71486-22-1 |
store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10 mg/mL (12.84 mM)
DMSO: 100 mg/mL (128.38 mM)
You can also refer to dose conversion for different animals. More
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Vinorelbine 71486-22-1 Cytoskeletal Signaling Microtubule Associated KW-2307 base inhibitor inhibit