Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vacquinol-1 is an MKK4 activator, which rapidly and selectively induces glioma cell death.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 195.00 | |
5 mg | In stock | $ 430.00 | |
10 mg | In stock | $ 636.00 | |
25 mg | In stock | $ 987.00 | |
50 mg | In stock | $ 1,360.00 | |
100 mg | In stock | $ 1,830.00 |
Description | Vacquinol-1 is an MKK4 activator, which rapidly and selectively induces glioma cell death. |
In vitro | Vacquinol-1 induces rapid and specific cell death of glioma cells with IC50 of 3.14 μM via membrane ruffling, cell rounding, massive macropinocytic vacuole accumulation, ATP depletion, and cytoplasmic membrane rupture. [1] |
In vivo | In mice bearing U3013 mg tumors, Vacquinol-1 (15 μM intracranial injection, or 20 mg/kg p.o.) attenuates tumor growth and prolonging survival. [1] |
Kinase Assay | 1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1]. |
Cell Research | Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer's instructions.(Only for Reference) |
Synonyms | Vacquinol 1 |
Molecular Weight | 352.86 |
Formula | C21H21ClN2O |
CAS No. | 5428-80-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 70 mg/mL (164.4 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Vacquinol-1 5428-80-8 MAPK JNK NSC 13316 hepatocellular carcinoma NSC-13316 inhibit Inhibitor MKK4-specific activator Vacquinol1 induces apoptosis glioblastoma glioblastoma multiforme Vacquinol 1 HCC activates MAPK pathways NSC13316 inhibitor