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Immunology/Inflammation IL Receptor Suplatast tosilate

Suplatast tosilate

Catalog No. T0117   CAS 94055-76-2
Synonyms: IPD 1151T, Suplatast Tosylate

Suplatast Tosilate is a novel capsular anti-asthmatic drug. It ( IC50 above 100 μM) inhibits both IgE production, IL-4 and IL-5 synthesis.

Suplatast tosilate, CAS 94055-76-2
Pack Size Availability Price/USD Quantity
50 mg In stock 50.00
100 mg In stock 70.00
200 mg Inquiry 98.00
1 mL * 10 mM (in DMSO) In stock 50.00
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Purity 99.34%
Biological Description
Chemical Properties
Storage & Solubility Information
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Description Suplatast Tosilate is a novel capsular anti-asthmatic drug. It ( IC50 above 100 μM) inhibits both IgE production, IL-4 and IL-5 synthesis.
Targets&IC50 Th2 cytokine
Kinase Assay ssay for cytokines: The cultures for cytokine production are set up at 37 °C as follows: the mixtures of SN-4 and autologous APC (each 1 × 105 cells/well) are cultured for 3 days with 50 μg/mL of Cry j1 in a total volume of 0.2 mL in round-bottomed micro plates; PBMC (2 × 10 5 cells/well) from normal donors are cultured for 24 hours with 10 μg/ml of PHA in a total volume of 0.2 mL in flat-bottomed, 96-well micro plates; and purified T cells (1 × 105 cells/well) from normal donors are cultured in a total volume of 1 ml for 24 hours with anti-CD3 mAb that have been immobilized on flat-bottomed, 24-well plates at a concentration of 5 µg/ml. Cytokines in the culture supernatants are quantitatively assayed by the following commercially available kits: IL-4 and IFN-γ. The sensitivity of the assay is 30 pg/mL for IL-4 and 1 U/mL for IFN-γ.
Cell Research
Allogeneic responder CD4+ T cells obtained from healthy subjects are purified from PBMCs by negative depletion of CD14+, CD16+, CD19+, CD56+, and CD8+ cells using magnetic cell separation system micro-beads and columns. After 7 days of culture with GM-CSF and IL-4, iDCs differentiated from monocytes in the presence or absence of Suplatast tosilate (10 and 100 mg/ mL) are co-cultured with purified 1×10 5 allogeneic CD41 T cells at varying ratios of iDCs in 96-well round bottomed culture plates for 5 days. Cells are pulsed with 1 μCi of 3H-methylthymidine for the last 8 hours of the culture period. Incorporated radioactivity is counted with a liquid scintillation counter, and proliferative responses are expressed as the mean 3 H-methylthymidine incorporation (counts per minutes) of triplicate wells_SEM. Proliferation of DCs alone or CD4+ T cells alone is also assessed.(Only for Reference)
Cell lines: Dendritic cells (DCs) and CD4+ T cells
Synonyms IPD 1151T , Suplatast Tosylate
Purity 99.34%
Molecular Weight 499.64
Formula C23H33NO7S2
CAS No. 94055-76-2

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 50 mg/mL (100 mM)

water: 50 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

Saline: 30 mg/mL

Citations

References and Literature
1. Mimura T, et al. J Pharmacol Exp Ther, 2005, 314(1), 293-301. 2. Yanagihara Y, et al. Jpn J Pharmacol, 1993, 61(1), 31-39. 3. Zhao GD, et al. Int Arch Allergy Immunol, 2000, 121(2), 116-122. 4. Tanaka A, et al. Clin Exp Allergy, 2007, 37(7), 1083-1089.

Related compound libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Histamine & Melatonin Library Immunology/Inflammation Compound Library Cytokine Inhibitor Library Anti-cancer Approved drug Library Anti-cancer Clinical Compound Library Anti-obesity Compound Library Anti-cancer Drug library Drug Repurposing Library HIF-1 Signaling Pathway Compound Library Nonsteroidal Antiinflammatory Compound Library NMPA-Approved Drug Library FDA Approved & Pharmacopeial Drug Library

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