Home Tools

NSC305787 hydrochloride

Catalog No. T12263L CAS 53868-26-1

Synonyms: (Rac)-NSC305787 hydrochloride

NSC305787 hydrochloride ((Rac)-NSC305787 hydrochloride is a cell membrane permeable and selective and potent small molecule dual inhibitor of Cdc25 dual specificity phosphatase and EZR that displays antitumor activity in pancreatic cancer cells and inhibits Cdc25B2, Cdc25A, Cdc25B2 and Cdc25C.NSC 663284 inhibits NSD2 (IC50 of 170 nM) enzyme activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

NSC305787 hydrochloride Chemical Structure

NSC305787 hydrochloride, CAS 53868-26-1

Pack Size	Availability	Price/USD	Quantity
1 mg	In stock	$ 154.00
5 mg	In stock	Inquiry

Bulk Inquiry

Select Batch

Purity: 99.07%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	NSC305787 hydrochloride ((Rac)-NSC305787 hydrochloride is a cell membrane permeable and selective and potent small molecule dual inhibitor of Cdc25 dual specificity phosphatase and EZR that displays antitumor activity in pancreatic cancer cells and inhibits Cdc25B2, Cdc25A, Cdc25B2 and Cdc25C.NSC 663284 inhibits NSD2 (IC50 of 170 nM) enzyme activity.
Targets&IC50	Ezrin:8.3 μM, Radixin:55 μM, PKCΙ:172.4 μM (Kd), MBP:58.9 μM, Ezrin:5.85 μM (Kd), Moesin:9.4 μM
In vitro	NSC305787 hydrochloride, an inhibitor of ezrin, exhibits a Kd of 5.85 μM and demonstrates antitumor activity. With IC50 values of 8.3, 9.4, 55, and 58.9 μM, NSC305787 hydrochloride inhibits PKCΙ phosphorylation of Ezrin, Moesin, Radixin, and MBP, respectively. Binding to PKCΙ with a Kd value of 172.4 μM, NSC305787 hydrochloride primarily inhibits ezrin T567 phosphorylation through its binding to ezrin, not via the inhibition of PKCΙ kinase activity. In the context of K7M2 osteosarcoma (OS) cells, NSC305787 hydrochloride (1, 10 μM) displays inhibitory activity against ezrin-mediated invasion. Furthermore, at a concentration of 10 μM, NSC305787 hydrochloride diminishes cell motility phenotypes in zebrafish and hinders OS metastatic growth in lung organ culture[2].
In vivo	In a mouse model,NSC305787 hydrochloride (i.p., 0.240 mg/kg/day) effectively suppresses ezrin-dependent osteosarcoma metastatic growth in the lung[1]. Additionally, in a transgenic mouse model of osteosarcoma (Osx-Cre+p53fl/flpRBfl/fl), NSC305787 hydrochloride (i.p., 240 μg/kg) exhibits a remarkable inhibition of pulmonary metastasis. Furthermore, it demonstrates a more favorable pharmacokinetic profile compared to NSC668394 in the same mouse model[1].

Synonyms	(Rac)-NSC305787 hydrochloride
Molecular Weight	481.89
Formula	C25H31Cl3N2O
CAS No.	53868-26-1

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 5 mg/mL(10.38 mM), Sonification is recommended.

H2O: <0.1 mg/mL(Insoluble)

References and Literature

1. Çelik H, et al. Ezrin Inhibition Up-regulates Stress Response Gene Expression. J Biol Chem. 2016 Jun 17;291(25):13257-70. 2. Bulut G, et al. Small molecule inhibitors of ezrin inhibit the invasive phenotype of osteosarcoma cells. Oncogene. 2012 Jan 19;31(3):269-81.

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

NSC305787 hydrochloride 53868-26-1 Others (Rac)-NSC305787 hydrochloride NSC305787 Hydrochloride NSC-305787 Hydrochloride NSC-305787 hydrochloride NSC 305787 Hydrochloride inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.