Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 64.00 | |
10 mg | In stock | $ 93.00 | |
25 mg | In stock | $ 197.00 | |
50 mg | In stock | $ 328.00 | |
100 mg | In stock | $ 563.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 48.00 |
Description | NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively). |
Targets&IC50 | CDC25A:32nM(ki), Cdc25C:40 nM(ki), Cdc25B:96nM(ki) |
In vitro | NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases[1]. |
Cell Research | To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates.?After overnight culture, NSC 95397 cells were added at the indicated concentrations.?After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit.?O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD.?Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1] |
Molecular Weight | 310.39 |
Formula | C14H14O4S2 |
CAS No. | 93718-83-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mg/mL (322.18 mM)
You can also refer to dose conversion for different animals. More
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NSC 95397 93718-83-3 Apoptosis Metabolism Phosphatase NSC-95397 Inhibitor inhibit NSC95397 inhibitor