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Metabolism Phosphatase NSC 95397

NSC 95397

Catalog No. T7078   CAS 93718-83-3

NSC 95397 is a  potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively).

NSC 95397, CAS 93718-83-3
Pack Size Availability Price/USD Quantity
1 mg In stock 24.00
5 mg In stock 72.00
10 mg In stock 104.00
25 mg In stock 230.00
50 mg In stock 410.00
100 mg In stock 734.00
1 mL * 10 mM (in DMSO) In stock 54.00
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Purity 97.12%
Biological Description
Chemical Properties
Storage & Solubility Information
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Description NSC 95397 is a  potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively).
Targets&IC50 Cdc25A ,   Cdc25B ,   Cdc25C
In vitro NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases[1].
Cell Research
To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates. After overnight culture, NSC 95397 cells were added at the indicated concentrations. After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit. O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1] 
Molecular Weight 310.39
Formula C14H14O4S2
CAS No. 93718-83-3

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 100 mg/mL (322.18 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

Citations

References and Literature
1. Navneet D , Bou-Yue P , Chien-Min L , et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-. 2. Park H , Carr B I , Li M , et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Apoptosis Compound Library Kinase Inhibitor Library Anti-cancer Metabolism Compound Library Phosphatase Inhibitor Library Lipid Metabolism Compound Library Glycometabolism Compound Library

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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.