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MMAF-OMe

Name: MMAF-OMe
Brand: TargetMol
SKU: T12081
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12081Cas No. 863971-12-4

Alias Monomethyl auristatin F methyl ester

MMAF-OMe (Monomethyl auristatin F methyl ester) is an antitubulin agent which inhibits several tumor cell lines with IC50s of 0.056 nM, 0.166 nM, 0.183 nM, and 0.449 nM for MDAMB435/5T4, MDAMB361DYT2, MDAMB468, and Raji (5T4-) cell lines, respectively. MMAF-Ome is also an ADC cytotoxin.

MMAF-OMe

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Purity: 99.34%

Catalog No. T12081Alias Monomethyl auristatin F methyl esterCas No. 863971-12-4

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$34	In Stock	In Stock
10 mg	$52	In Stock	In Stock
25 mg	$82	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.34%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	MMAF-OMe (Monomethyl auristatin F methyl ester) is an antitubulin agent which inhibits several tumor cell lines with IC50s of 0.056 nM, 0.166 nM, 0.183 nM, and 0.449 nM for MDAMB435/5T4, MDAMB361DYT2, MDAMB468, and Raji (5T4-) cell lines, respectively. MMAF-Ome is also an ADC cytotoxin.
In vitro	2.5F-Fc and 2.5F-Fc-MMAF exhibit similar IC50 values (6.9±1.1 vs. 8.3±1.3 nM, respectively)[1], indicating that MMAF conjugation has a negligible impact on integrin-binding affinity[1].
Synonyms	Monomethyl auristatin F methyl ester

Chemical Properties

Molecular Weight	745.99
Formula	C₄₀H₆₇N₅O₈
Cas No.	863971-12-4
Smiles	CC[C@H](C)[C@@H]([C@@H](CC(=O)N1CCC[C@H]1[C@H](OC)[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)OC)OC)N(C)C(=O)[C@@H](NC(=O)[C@@H](NC)C(C)C)C(C)C
Relative Density.	1.095 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 99 mg/mL (132.71 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+95% Saline: 4 mg/mL (5.36 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3405 mL	6.7025 mL	13.4050 mL	67.0250 mL
5 mM	0.2681 mL	1.3405 mL	2.6810 mL	13.4050 mL
10 mM	0.1341 mL	0.6703 mL	1.3405 mL	6.7025 mL
20 mM	0.0670 mL	0.3351 mL	0.6703 mL	3.3513 mL
50 mM	0.0268 mL	0.1341 mL	0.2681 mL	1.3405 mL
100 mM	0.0134 mL	0.0670 mL	0.1341 mL	0.6703 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc