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Cytoskeletal Signaling Wnt/beta-catenin IWP2

IWP2

Catalog No. T2702   CAS 686770-61-6
Synonyms: IWP 2, IWP-2

IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.

IWP2, CAS 686770-61-6
Pack Size Availability Price/USD Quantity
5 mg In stock 60.00
10 mg In stock 88.00
25 mg In stock 180.00
50 mg In stock 330.00
100 mg In stock 462.00
200 mg Inquiry 650.00
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Purity 96.10%
Purity 99.27%
Biological Description
Chemical Properties
Storage & Solubility Information
Description IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.
Targets&IC50 Wnt
Cell Research
IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera[2].
Synonyms IWP 2 , IWP-2
Purity 96.10%
Molecular Weight 466.6
Formula C22H18N4O2S3
CAS No. 686770-61-6

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 2mg/ml(4.29mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Chen B, et al. Nat Chem Biol, 2009, 5(2), 100-107. 2. Maiti G, et al. The Wingless homolog Wnt5a stimulates phagocytosis but not bacterial killing. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16600-5. 3. Liu JD, et al. Wnt/Glycogen Synthase Kinase 3β/β-catenin Signaling Activation Mediated Sevoflurane Preconditioning-induced Cardioprotection. Chin Med J (Engl). 2015 Sep 5;128(17):2346-53. 4. García-Reyes B, et al. Discovery of Inhibitor of Wnt Production 2 (IWP-2) and Related Compounds As Selective ATP-Competitive Inhibitors of Casein Kinase 1 (CK1) δ/ε. J Med Chem. 2018 May 10;61(9):4087-4102.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Stem cell Differentiation Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library Wnt&Hedgehog&Notch Compound Library Promoting Cancer Cell Differentiation Compound Library Anti-obesity Compound Library Neural Regeneration Compound Library Osteogenesis-related Compound Library Anti-aging Compound Library Target-Focused Phenotypic Screening Library Anti-Breast Cancer Compound Library Cytoskeletal Signaling Pathway Compound Library Anti-fibrosis Compound Library

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