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HTH-01-015

Catalog No. T2374 CAS 1613724-42-7

HTH-01-015 is a selective NUAK1 inhibitor (IC50=100 nM).

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HTH-01-015, CAS 1613724-42-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 37.00
2 mg	In stock	$ 50.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 108.00
25 mg	In stock	$ 223.00
50 mg	In stock	$ 318.00
100 mg	In stock	$ 428.00
1 mL * 10 mM (in DMSO)	In stock	$ 79.00

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Purity: 99.08%

Purity: 98.62%

Purity: 98.38%

Purity: 98.19%

Purity: 98.1%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	HTH-01-015 is a selective NUAK1 inhibitor (IC50=100 nM).
Targets&IC50	NUAK1:100 nM
In vitro	In HEK-293 cells express NUAK1 as well as NUAK2, HTH-01-015 suppresses NUAK1-mediated MYPT1 phosphorylation. HTH-01-015 suppresses cell migration In NUAK1+/+ MEFs, and inhibit U2OS cell invasion. Moreover, HTH-01-015 inhibits cell proliferation in both cell lines. [1] HTH-01-015 inhibitors markedly restricted cells from entering into mitosis in U2OS cells. [2]
Kinase Assay	Kinase activity assays: In vitro activities of purified GST–NUAK1 and GST–NUAK1[A195T] are measured using Cerenkov counting of incorporation of radioactive 32P from [γ-32P]ATP into Sakamototide substrate peptide. Reactions are carried out in a 50 μL reaction volume for 30 min at 30°C and reactions are terminated by spotting 40 μL of the reaction mix on to P81 paper and immediately immersing in 50 mM orthophosphoric acid. Samples are washed three times in 50 mM orthophosphoric acid followed by a single acetone rinse and air drying. The kinase-mediated incorporation of [γ-32P]ATP into Sakamototide is quantified by Cerenkov counting. One unit of activity is defined as that which catalyses the incorporation of 1 nmol of [32P]phosphate into the substrate over 1 h.
Cell Research	Cell proliferation assays are carried out colorimetrically in 96-well plates using the CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay kit following the manufacturer's protocol. Initially, 2000 cells per well are seeded for U2OS cells and 3000 cells per well are seeded for MEFs. The proliferation assays are carried out over 5 days in the presence or absence of 10 μM HTH-01-015.(Only for Reference)

Molecular Weight	468.55
Formula	C26H28N8O
CAS No.	1613724-42-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 54 mg/mL (115.2 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 28 mg/mL (59.8 mM)

References and Literature

1. Banerjee S, et al. Biochem J. 2014, 457(1), 215-225. 2. Banerjee S, et al. Biochem J. 2014, 461(2), 233-245.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Highly Selective Inhibitor Library Inhibitor Library Bioactive Compound Library Anti-Breast Cancer Compound Library Antidepressant Compound Library Anti-Lung Cancer Compound Library NO PAINS Compound Library Anti-Cancer Compound Library Anti-Liver Cancer Compound Library

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Keywords

HTH-01-015 1613724-42-7 Chromatin/Epigenetic PI3K/Akt/mTOR signaling AMPK inhibit AMP-activated protein kinase HTH 01 015 HTH01015 Inhibitor inhibitor

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