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H2DCFDA

Catalog No. T15458 CAS 4091-99-0

Synonyms: 2',7'-Dichlorodihydrofluorescein diacetate, DCFH-DA

H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.

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H2DCFDA, CAS 4091-99-0

Pack Size	Availability	Price/USD
50 mg	In stock	$ 37.00
100 mg	In stock	$ 50.00
1 mL * 10 mM (in DMSO)	In stock	$ 30.00

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Purity: 99.47%

Purity: 98.06%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.
In vitro	METHODS: Flow cytometry was used to detect ROS levels: 1, H2DCFDA was dissolved into 10 mM DMSO stock solution and further diluted with PBS before use. 2. Adherent cells are incubated with 5 µM H2DCFDA solution for 30 min at 37°C, protected from light, then harvested with 0.05% trypsin-EDTA solution, suspended in fresh medium and immediately analyzed by flow cytometry (488 nm). [1] METHODS: Confocal microscopy was performed to detect ROS levels: 1. H2DCFDA was dissolved into 10 mM DMSO stock solution and further diluted with PBS before use. 2. Coverslips containing cells were placed in 5 µM H2DCFDA staining solution and incubated for 60 min at 37°C, protected from light, then washed and imaged with a confocal laser scanning microscope Leica TCS SL equipped with an argon laser. [1]
In vivo	METHODS: Fluorescence microscopy was used to analyze the oxidative activity of LPS induced peritonitis in mice: 1, H2DCFDA was dissolved in 100 µL ethanol and further diluted with PBS before use. 2. C57BL/6J mice were injected intraperitoneally with LPS (0.1-1 mg/mL) to induce peritonitis. 3.5 h later, H2DCFDA (0.1-0.8 mg/ml) was injected intraperitoneally. 3. 30 min after H2DCFDA injection, the animals were killed by cervical dislocation, and the peritoneal cells were recovered by rinsing with 5 mL of ice-cold HBSS solution at pH 7.4. 4. Peritoneal cells were washed and resuspended in PBS. Macrophages were removed by adhesion method after incubation in a polystyrene dish at 37℃ for 30 min. The supernatant was recovered and approximately 20,000-25,000 leukocytes were added to microscope slides using a cytocentrifuge. The slides were then analyzed using fluorescence microscopy. [2]

Synonyms	2',7'-Dichlorodihydrofluorescein diacetate, DCFH-DA
Molecular Weight	487.29
Formula	C24H16Cl2O7
CAS No.	4091-99-0

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 14.29 mg/mL (29.33 mM), Sonication is recommended.

DMSO: 150 mg/mL (307.82 mM)

References and Literature

1. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769. 2. Linda S, et al. Method for analysis of intracellular production of free radicals in vivo. SWEDISH DEFENCE RESEARCH AGENCY. 2001 Mar.

Citations

1. Zhang J, Liu P, Chen J, et al. Upgrade of chrysomycin A as a novel topoisomerase II inhibitor to curb KRAS-mutant lung adenocarcinoma progression. Pharmacological Research. 2022: 106565. 2. Hu Y, Wen Q, Cai Y, et al. Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway. Phytomedicine. 2022: 154528. 3. Zhang Y, Cai Y, Wang T, et al.A common tolerance mechanism of bacterial biofilms to antibiotics.bioRxiv.2023: 2023.01. 30.526163. 4. Pan Y, Wang X, Tan Q, et al.Effects and Mechanisms of Prussian Blue Nanozymes with Multiple Enzyme Activities on Nasopharyngeal Carcinoma Cells.Tissue and Cell.2024: 102316. 5. Guo Y, Zhu L, Duan Y, et al.Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission.Cell Death & Disease.2024, 15(2): 125. 6. Zhou X, Qin M, He L, et al.Geraniin restricts inflammasome activation and macrophage pyroptosis by preventing the interaction between ASC and NLRP3 to exert anti-inflammatory effects.International Immunopharmacology.2024, 129: 111656. 7. Yong Y Y, Yan L, Wang B D, et al.Penthorum chinense Pursh inhibits ferroptosis in cellular and Caenorhabditis elegans models of Alzheimer's disease.Phytomedicine.2024: 155463.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Pyroptosis Compound Library NO PAINS Compound Library Cuproptosis Compound Library Anti-Liver Cancer Compound Library Ferroptosis Compound Library Mitochondria-Targeted Compound Library Anti-Prostate Cancer Compound Library Anti-Obesity Compound Library Oxidation-Reduction Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Keywords

H2DCFDA 4091-99-0 Immunology/Inflammation Metabolism NF-Κb Reactive Oxygen Species inhibit 2',7'-Dichlorodihydrofluorescein Diacetate Inhibitor H-2DCFDA 2',7'-Dichlorodihydrofluorescein 2',7'-Dichlorodihydrofluorescein diacetate DCFH-DA inhibitor

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