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Guanidine hydrochloride

Catalog No. T0415 CAS 50-01-1

Synonyms: Guanidine HCl, Aminoformamidine HCl, Aminoformamidine Hydrochloride, Guanidinium chloride

Guanidine hydrochloride (Aminoformamidine Hydrochloride) is a strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant.

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Guanidine hydrochloride Chemical Structure

Guanidine hydrochloride, CAS 50-01-1

Pack Size	Availability	Price/USD	Quantity
10 g	In stock	$ 47.00
1 mL * 10 mM (in DMSO)	In stock	$ 26.00

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Purity: 99.68%

Biological Description

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Description

In vitro

Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. [1] Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state. [2] Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GWaterapp. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. [3] Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast. [4] 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. [5]

Synonyms	Guanidine HCl, Aminoformamidine HCl, Aminoformamidine Hydrochloride, Guanidinium chloride
Molecular Weight	95.53
Formula	CH6ClN3
CAS No.	50-01-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 198.9 mM

H2O: 198.9 mM

References and Literature

1. Pace CN. Methods Enzymol, 1986, 131, 266-280. 2. Hagihara Y, et al. J Mol Biol, 1993, 231(2), 180-184. 3. Greene RF Jr, et al. J Biol Chem, 1974, 249(17), 5388-5393. 4. Eaglestone SS, et al. Proc Natl Acad Sci U S A, 2000, 97(1), 240-244. 5. Jung G, et al. Curr Microbiol, 2001, 43(1), 7-10.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Drug Repurposing Compound Library Traditional Chinese Medicine Monomer Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Human Endogenous Metabolite Library Clinical Compound Library RO5 Drug-like Natural Product Library Anti-Infection Compound Library

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Keywords

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