Angiogenesis Bcr-Abl GNF2


Catalog No. T1817   CAS 778270-11-4
Synonyms: GNF 2, GNF-2

GNF-2 is a highly selective non-ATP competitive inhibitor of Bcr-Abl.

GNF2, CAS 778270-11-4
Pack Size Availability Price/USD Quantity
2 mg In stock 48.00
5 mg In stock 68.00
10 mg In stock 113.00
25 mg In stock 227.00
50 mg In stock 410.00
100 mg In stock 680.00
1 mL * 10 mM (in DMSO) In stock 74.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description GNF-2 is a highly selective non-ATP competitive inhibitor of Bcr-Abl.
Targets&IC50 Bcr-Abl (K562 cell line) : ic50 273nM,   Bcr-Abl (SUP-B15 cell line) : ic50 268nM,  
Kinase Assay Binding assay: Recombinant proteins (100 nM for each construct) or immunoprecipitated proteins are diluted in kinase buffer (20 mM HEPES (pH 7.4), 50 mM KCl, 0.1% CHAPS, 30 mM MgCl2, 2 mM MnCl2, 1 mM DTT, and 1% glycerol). Aliquots of the diluted proteins are preincubated with either DMSO or compounds for 30 min at room temperature and then added to K-LISA PTK EAY reaction plates. The kinase reaction is initiated by adding 0.1 mM ATP and is allowed to proceed for 30 min at room temperature. The phosphorylation of GST-Abltide is monitored by SDS-PAGE and phosphorimaging analysis or autoradiography.
Cell Research
Cells (0.3-0.6 × 106 per mL) are plated in duplicate or triplicate in 96-well plates containing increasing GNF-2 concentrations (5 nM–10 μM). After incubation at 37 ℃ in 5% CO2 for 48 hours, the effect of GNF-2 on cell viability is determined by the MTT colorimetric dye reduction method. Inhibition of cell proliferation is calculated as a percentage of growth of DMSO-treated cells, and IC50 values are determined with Microsoft Excel XLfit3.(Only for Reference)
Cell lines: Ba/F3.p210, Ba/F3.p210E255V and Ba/F3.p185Y253H cells
Synonyms GNF 2 , GNF-2
Purity 98.00%
Molecular Weight 374.32
Formula C18H13F3N4O2
CAS No. 778270-11-4


0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 37.4 mg/mL (100 mM)

Ethanol: 18.7 mg/mL (50 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )


References and Literature
1. Adrián FJ, et al. Nat Chem Biol, 2006, 2(2), 95-102 2. Choi Y, et al. J Biol Chem, 2009, 284(42), 292005-292014. 3. Zhang J, et al. Nature, 2010, 463(7280), 501-506. 4. Fabbro D, et al. Biochim Biophys Acta, 2010, 1804(3), 454-462.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Tyrosine kinase inhibitor library Kinase Inhibitor Library Fluorochemical Library Angiogenesis related Compound Library

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