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DNA Damage/DNA Repair ATM/ATR Chloroquine diphosphate

Chloroquine diphosphate

Catalog No. T0194   CAS 50-63-5
Synonyms: Aralen phosphate, Chingamin phosphate
Purity 99.79% Datasheet MSDS

Chloroquine is an aminoquinoline antimalarial and also is widely used as an autophagy inhibitor. Chloroquine also is an inhibitor of toll-like receptors (TLRs).

Chloroquine diphosphate, CAS 50-63-5
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50 mg In stock 50.00
100 mg In stock 64.00
200 mg In stock 108.00
500 mg In stock 212.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Chloroquine is an aminoquinoline antimalarial and also is widely used as an autophagy inhibitor. Chloroquine also is an inhibitor of toll-like receptors (TLRs).
Targets&IC50 ATM,  
In vivo Chloroquine (80 mg/kg, i.p.) does not prevent the growth of the triple-negative MDA-MB-231 cells with high or low TLR9 expression levels in the orthotopic mouse model[4]. TLR7 and TLR9 inhibition using IRS-954 or chloroquine significantly inhibits tumour growth in the mouse xenograft model. HCC development in the DEN/NMOR rat model is also significantly inhibited by chloroquine[5].
Cell Research
The cells are cultured in 6-well plates with normal culture medium in the presence of vehicle or 25 or 50 μM chloroquine, until near confluency, after which they are rinsed with sterile phosphate-buffered saline (PBS) and cultured further for the indicated times in serum-free culture medium. At the desired time-points, the culture medium is discarded and the cells are quickly harvested in lysis buffer and clarified by centrifugation. Subsequent to boiling the supernatants in reducing sodium dodecyl sulphate (SDS) sample buffer, equal amounts of protein (100 μg) are loaded per lane and the samples are electrophoresed into 10 or 4-20% gradient polyacrylamide SDS gels, then transferred to a nitrocellulose membrane. To detect TLR9, the blots are incubated overnight at 4°C with anti-TLR9 antibodies, diluted 1:500 in Tris-buffered saline with 0.1% (v/v) Tween-20 (TBST). Equal loading is confirmed with polyclonal rabbit anti-actin. Secondary detection is performed with horseradish peroxidase-linked secondary antibodies. The protein bands are visualized by chemiluminescence using an ECL kit.
Animal Research
Animal Model: Chloroquine diphosphate is dissolved in PBS
Synonyms Aralen phosphate, Chingamin phosphate
Purity 98.00%
Appearance solid
Molecular Weight 515.87
Formula C18H26CLN3·2(H3PO4)
CAS No. 50-63-5

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

H2O: 33 mg/mL (63.97 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Said A, et al. Chloroquine promotes IL-17 production by CD4+ T cells via p38-dependent IL-23 release by monocyte-derived Langerhans-like cells. J Immunol. 2014 Dec 15;193(12):6135-43. 2. Tuomela J, et al. Chloroquine has tumor-inhibitory and tumor-promoting effects in triple-negative breast cancer. Oncol Lett. 2013 Dec;6(6):1665-1672. 3. Mohamed FE, et al. Effect of toll-like receptor 7 and 9 targeted therapy to prevent the development of hepatocellular carcinoma. Liver Int. 2014 Jul 2. doi: 10.1111/liv.12626.

Related Compound Libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Autophagy Compound Library PI3K-AKT-mTOR Compound Library FDA-approved Drug Library Immunology/Inflammation Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library Anti-cancer Approved drug Library Cardiotoxicity Compound Library Killers Collection

Related Products

Related compounds with same targets
Chloroquine diphosphate CGK 733 AZ20 ETP46464 Dactolisib NU7026 KU-60019 PIK-93

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