Home Tools
Log in
Cart

5a-Pregnane-3,20-dione

Catalog No. T5556   CAS 566-65-4

5a-Pregnane-3,20-dione is a biologically active 5-alpha-reduced metabolite of plasma progesterone

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
5a-Pregnane-3,20-dione Chemical Structure
5a-Pregnane-3,20-dione, CAS 566-65-4
Pack Size Availability Price/USD Quantity
100 mg In stock $ 39.00
1 mL * 10 mM (in DMSO) In stock $ 39.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 97.01%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description 5a-Pregnane-3,20-dione is a biologically active 5-alpha-reduced metabolite of plasma progesterone
In vitro the endogenous progesterone metabolite,5a-Pregnane-3,20-dione, may be involved in promoting breast neoplasia and metastasis by affecting adhesion and cytoskeletal molecules.
Cell Research MCF-7 cells were grown without or with 5a-Pregnane-3,20-dione (10(-9)-10(-5) M), and the effects on cell and nuclear morphology, adhesion plaques, vinculin and actin expression, actin polymerization, and microfilament distribution were examined by immunohistochemistry, morphometry (using confocal microscopy and digital computer imaging analysis), and Western blotting.?Treatment of cells with 10(-9)-10(-6) M 5a-Pregnane-3,20-dione resulted in dose-dependent decreases in cell area, cell-to-cell contacts, and attachment to the substratum, and increases in variation in nuclear area.?These changes in the 5alphaP-treated cells were accompanied by decreases in vinculin-containing adhesion plaques, vinculin expression, polymerized actin stress fibers, and decreases in insoluble and increases in soluble actin fractions.
Molecular Weight 316.48
Formula C21H32O2
CAS No. 566-65-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 5 mg/mL (15.80 mM), sonification/heating is recommended.

TargetMolReferences and Literature

1. Wiebe J P , Muzia D . The endogenous progesterone metabolite, 5a-pregnane-3,20-dione, decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerized F-actin in MCF-7 breast cancer cells.[J]. Endocrine, 2001, 16(1):7-14.

Related compound libraries

This product is contained In the following compound libraries:
Human Endogenous Metabolite Library Human Metabolite Library Anti-Tumor Natural Product Library Endocrinology-Hormone Compound Library RO5 Drug-like Natural Product Library Natural Product Library for HTS Natural Product Library Bioactive Compounds Library Max Human Endogenous Metabolite Compound Library Plus Mouse Metabolite Compound Library

Related Products

Related compounds with same targets
1-Methylxanthine Glycerol 3-phosphate biscyclohexylammonium salt DL-5-Hydroxylysine hydrochloride Tauroursodeoxycholate trans-Cinnamaldehyde Ellagic acid 6-Hydroxypyridin-2(1H)-one hydrochloride L-Alloisoleucine

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

5a-Pregnane-3,20-dione 566-65-4 Metabolism Endogenous Metabolite Inhibitor inhibit 5aPregnane3,20dione 5a Pregnane 3,20 dione inhibitor

 

TargetMol