Home Tools

3CAI

Catalog No. T14034 CAS 28755-03-5

3CAI is a potent and specific AKT1 and AKT2 inhibitor which showed significant inhibition of AKT in an in vitro kinase assay and suppressed expression of AKT direct downstream targets such as mTOR and GSK3β as well as induced growth inhibition and apoptosis in colon cancer cells.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

3CAI, CAS 28755-03-5

Pack Size	Availability	Price/USD
5 mg	In stock	$ 50.00
10 mg	In stock	$ 70.00
25 mg	In stock	$ 127.00
50 mg	In stock	$ 232.00
100 mg	In stock	$ 348.00
200 mg	In stock	$ 511.00
500 mg	In stock	$ 829.00
1 mL * 10 mM (in DMSO)	In stock	$ 58.00

Bulk Inquiry

Select Batch

Purity: 98.13%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	3CAI is a potent and specific AKT1 and AKT2 inhibitor which showed significant inhibition of AKT in an in vitro kinase assay and suppressed expression of AKT direct downstream targets such as mTOR and GSK3β as well as induced growth inhibition and apoptosis in colon cancer cells.
In vitro	3CAI, a potential AKT inhibitor, exhibits significantly higher potency in inhibiting AKT1 compared to PI3K (60% inhibition at 1 µM vs 10 µM, respectively). In experiments with HCT116 and HT29 colon cancer cells treated with 3CAI (4 µM), I3C, or an AKT inhibitor in 1% FBS/McCoy's 5A (for HCT116) over four days, 3CAI notably increased apoptotic cell numbers versus untreated controls. Additionally, 3CAI markedly reduced AKT-mediated phosphorylation of mTOR (Ser2448) and GSK3β (Ser9) in a time-dependent manner, while also elevating pro-apoptotic proteins p53 and p21 after 12 or 24 hours of treatment. In vitro kinase assays revealed that 3CAI solely inhibits AKT1 kinase activity without affecting the activities of MEK1, JNK1, ERK1, and TOPK at 1 µM. Its capability to suppress AKT1 and AKT2 activities indicates a dose-dependent effect, with a profound impact on downstream AKT targets and the induction of apoptosis.
In vivo	To assess the in vivo antitumor efficacy of 3CAI, athymic nude mice with HCT116 cancer cell-induced tumors on their right flank were orally administered 3CAI at doses of 20 or 30 mg/kg, I3C at 100 mg/kg, or a vehicle control, five times weekly for a duration of 21 days. The higher dosage of 3CAI (30 mg/kg) was found to notably suppress the expression of AKT-target proteins in tumor tissues. Furthermore, this dosage significantly reduced tumor growth by 50% compared to the control group (p<0.05), without inducing noticeable side effects or substantial weight loss in the mice, suggesting a good tolerance to the treatment.

Molecular Weight	193.63
Formula	C10H8ClNO
CAS No.	28755-03-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 246 mg/mL (1270.46 mM), Sonification is recommended.

References and Literature

1. Kim DJ, et al. (3-Chloroacetyl)-indole, a novel allosteric AKT inhibitor, suppresses colon cancer growth in vitro and in vivo. Cancer Prev Res (Phila). 2011 Nov;4(11):1842-51.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Breast Cancer Compound Library Anti-Cancer Metabolism Compound Library Antidepressant Compound Library Anti-Ovarian Cancer Compound Library Bioactive Compound Library Neuronal Differentiation Compound Library Cytoskeletal Signaling Pathway Compound Library Bioactive Compounds Library Max Anti-Obesity Compound Library

Related Products

Related compounds with same targets

Kihadanin B M2698 SC79 Chromeceptin Glaucocalyxin A CCT128930 Tubulin/AKT1-IN-1 Esculetin

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

3CAI 28755-03-5 Cytoskeletal Signaling PI3K/Akt/mTOR signaling Akt Inhibitor inhibit Protein kinase B PKB inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.