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3BDO

Catalog No. T14033 CAS 890405-51-3

3BDO is a new mTOR activator. 3BDO inhibits autophagy.

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3BDO, CAS 890405-51-3

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 63.00
10 mg	In stock	$ 98.00
25 mg	In stock	$ 219.00
50 mg	In stock	$ 349.00
100 mg	In stock	$ 547.00
200 mg	In stock	$ 817.00
500 mg	In stock	$ 1,250.00
1 mL * 10 mM (in DMSO)	In stock	$ 68.00

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Purity: 96.00%

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Description	3BDO is a new mTOR activator. 3BDO inhibits autophagy.
In vitro	Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced with rapamycin. 3BDO also inhibits the effect of rapamycin in HUVECs. The phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses the phosphorylation. The results show that 3BDO suppresses the increased MAP1LC3B puncta number, MAP1LC3B-II level and decreased SQSTM1 protein level induced by rapamycin. 3BDO could dose- and time-dependently decrease FLJ11812 level in HUVECs. Overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO[1].
In vivo	Immunofluorescence assay demonstrates that treatment with 3BDO enhances p-p70S6K levels while reducing ATG13 protein levels in the plaque endothelium of mice, without affecting the phosphorylation of mTOR's immediate downstream targets p70S6K and 4EBP1. Comparatively, in apoE-/- mice, 3BDO treatment notably inhibits endothelium autophagy and apoptosis, thereby offering protection against endothelial injury in atherosclerosis. Furthermore, 3BDO treatment contributes to the stabilization of established atherosclerotic lesions in apoE-/- mice and results in a significant reduction in the serum levels of IL-6 and IL-8.

Molecular Weight	327.33
Formula	C18H17NO5
CAS No.	890405-51-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 150 mg/mL (458.25 mM)

Ethanol: 20 mg/mL (61.10 mM), Sonication is recommended.

References and Literature

1. Ge D, et al. Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells. Autophagy. 2014 Jun;10(6):957-71. 2. Peng N, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E?/? mice. Sci Rep. 2014 Jul 1;4:5519.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Immunology/Inflammation Compound Library Anti-Aging Compound Library Anti-Cancer Compound Library Anti-Liver Cancer Compound Library Covalent Inhibitor Library Glycolysis Compound Library Autophagy Compound Library Antioxidant Compound Library Metabolism Compound Library

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Keywords

3BDO 890405-51-3 Apoptosis Autophagy PI3K/Akt/mTOR signaling mTOR Mammalian target of Rapamycin Inhibitor inhibit inhibitor

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