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PI3K/Akt/mTOR signaling PI3K 3-Methyladenine

3-Methyladenine

Catalog No. T1879   CAS 5142-23-4
Synonyms: 3-MA, NSC 66389
Purity 99.51% Datasheet MSDS

3-Methyladenine (3-MA) is a selective PI3KV inhibitor, and the IC50s against ps34 and PI3Kγ were 25/60 μM in HeLa cells, respectively.

3-Methyladenine, CAS 5142-23-4
Pack Size Availability Price/USD Quantity
25 mg In stock 42.00
50 mg In stock 59.00
100 mg In stock 91.00
200 mg In stock 156.00
500 mg In stock 296.00
1 mL * 10 mM (in DMSO) In stock 50.00
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Biological Description
Chemical Properties
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Description 3-Methyladenine (3-MA) is a selective PI3KV inhibitor, and the IC50s against ps34 and PI3Kγ were 25/60 μM in HeLa cells, respectively.
Targets&IC50 PI3Kγ :ic50 60μM,   Vps34 :ic50 25μM,  
In vivo In severe acute pancreatitis (SAP) group, the pathological change increased with time after modeling. Pathological change of the pancreas tissue in 3-methyladenine group was milder than those in the SAP group at both 12 and 24h [4]. 3-MA pretreatment significantly aggravated neurological symptoms when compared with the SAH + vehicle group. a large number of dying neurons from the SAH + 3-MA group showed cell shrinkage, chromatin condensation at the nuclear membrane and nuclear and cellular fragmentation, which suggest that the neurons were undergoing apoptotic cell death [5].
Kinase Assay Protein degradation assay: HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research
Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer. (Only for Reference)
Cell lines: HeLa cell line
Animal Research
Animal Model: Adult Sprague–Dawley rats weighing 300-350 g
Synonyms 3-MA, NSC 66389
Purity 98.00%
Appearance solid
Molecular Weight 149.15
Formula C6H7N5
CAS No. 5142-23-4

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

Ethanol: 4 mg/mL (26.81 mM)

DMSO: 3 mg/mL (20.11 mM), warmed

Water: 8 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL

Citations

References and Literature
1. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7. 2. Canu N, et al. Role of the autophagic-lysosomal system on low potassium-induced apoptosis in cultured cerebellar granule cells. J Neurochem. 2005 Mar;92(5):1228-42. 3. Hou H, et al. Inhibitors of phosphatidylinositol 3'-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665. 4. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats. Mediators Inflamm. 2016;2016:8369704. 5. Jing CH, et al. Autophagy activation is associated with neuroprotection against apoptosis via a mitochondrial pathway in a rat model of subarachnoid hemorrhage. Neuroscience. 2012 Jun 28;213:144-53.

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Natural Compound Library for HTS Anti-cancer Compound Library Autophagy Compound Library PI3K-AKT-mTOR Compound Library Kinase Inhibitor Library

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