Powder: -20°C for 3 years | In solvent: -80°C for 1 year
4-Cyclohexyl-1-phenylthiosemicarbazide is employed as an intermediate.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 35.00 | |
2 mg | In stock | $ 48.00 | |
5 mg | In stock | $ 72.00 | |
10 mg | In stock | $ 93.00 | |
25 mg | In stock | $ 168.00 | |
50 mg | In stock | $ 249.00 |
Description | 4-Cyclohexyl-1-phenylthiosemicarbazide is employed as an intermediate. |
Kinase Assay | Recombinant ROCK-I, ROCK-II, PKN, or citron kinase is expressed in HeLa cells as Myc-tagged proteins by transfection using Lipofectamine, and is precipitated from the cell lysates by the use of 9E10 monoclonal anti-Myc antibody coupled to G protein-Sepharose. Recovered immunocomplexes are incubated with various concentrations of [32P]ATP and 10 mg of histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 30 min in a total volume of 30 μL of the kinase buffer containing 50 mM HEPES-NaOH, pH 7.4, 10 mM MgCl2, 5 mM MnCl2, 0.02% Briji 35, and 2 mM dithiothreitol. PKCa is incubated with 5 μM [32P]ATP and 200 μg/mL histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 10 min in a kinase buffer containing 50 mM Tris-HCl, pH 7.5, 0.5 mM CaCl2, 5 mM magnesium acetate, 25 μg/mL phosphatidyl serine, 50 ng/mL 12-O-tetradecanoylphorbol-13-acetate and 0.001% leupeptin in a total volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer. After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured[1]. |
Molecular Weight | 249.38 |
Formula | C13H19N3S |
CAS No. | 27421-91-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mM
You can also refer to dose conversion for different animals. More
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4-Cyclohexyl-1-phenylthiosemicarbazide 27421-91-6 Others 4 Cyclohexyl 1 phenylthiosemicarbazide 4Cyclohexyl1phenylthiosemicarbazide inhibitor inhibit