Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1-EBIO (1-EBIO) is a calium channel agonist.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 42.00 | |
100 mg | In stock | $ 58.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 45.00 |
Description | 1-EBIO (1-EBIO) is a calium channel agonist. |
Kinase Assay | Procedures for BTK OMNIA Assay: The Omnia continuous read assay is performed essentially as described by the vendor. The assay conditions are: 40 μM ATP (1X KMATP), 10 μM Y5-Sox, and 10 nM BTK enzyme. Briefly, a substrate mix containing 1.13X ATP and the Y5 Sox substrate is first prepared in 1X Omnia Kinase Reaction Buffer (KRB) consisting of 20 mM Tris, pH 7.5, 5 mM MgCl2, 1 mM EGTA, 5 mM?β-glycerophosphate, 5% glycerol, and 0.2 mM DTT. For IC50 measurements, 5 μL of enzyme are incubated with serially diluted (3-fold) compounds prepared in 50% DMSO in a Corning (#3574) 384-well, white, non-binding surface microtiter plate at 25°C for 30 min. Kinase reactions are started with the addition of 45 μL of the ATP/Y5 substrate mix and monitored at λex360/λem485 in a Synergy 4 plate reader for 60 minutes. Progress curves from each well are examined for linear reaction kinetics and fit statistics. Initial velocity from each reaction is determined from the slope of a plot of relative fluorescence units versus time and then plotted against inhibitor concentration to estimate IC50 using the Response, Variable Slope model in GraphPad Prism from GraphPad Software. |
Molecular Weight | 162.19 |
Formula | C9H10N2O |
CAS No. | 10045-45-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (369.93 mM)
You can also refer to dose conversion for different animals. More
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1-EBIO 10045-45-1 Membrane transporter/Ion channel Metabolism Calcium Channel inhibit KcsA 1Ethyl2benzimidazolinone Potassium Channel Inhibitor 1 Ethyl 2 benzimidazolinone inhibitor